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To evaluate OGDC activity, mitochondria were isolated and the -KG-dependent succinyl-CoA production rate in mitochondria lysate was measured using LCMS

It is a cofactor for lysyl oxidase, an enzyme required for cross-linking collagen and elastin, helping maintain strong tendons, ligaments, and bone matrix integrity

Cell viability assay Primary neurons, plated in 96-well plates (2,000 cells/well), were treated with EW at serial concentrations (0, 5, 10, 20, 40, 80, 160 g/mL) following cell adhesion for subsequent experiments [35]

The mechanisms responsible for garlic - drug interactions and their in vivo relevance

the full mixing walkthrough lives on the reconstitution guide